Associate Scientist, Biochemistry

  • BioNTech Careers
  • Cambridge, Massachusetts
  • Work experience
  • Research, development, teaching
  • Published: 20.05.2022
  • published till: 31.07.2022
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Associate Scientist, Biochemistry

Become Part of the BioNTech Family.

Based in Cambridge, MA, BioNTech US serves as BioNTech’s North American headquarters and is an important part of our global effort to pioneer the development of next-generation immuno-oncology therapies. BioNTech US is a fully integrated subsidiary with a strong foundation in Europe and a focus on the development of novel neoantigen-targeted T cell therapies, complementing BioNTech’s highly innovative scientific approach and diversified pipeline of transformative cancer medicines. As a key research and clinical development hub, BioNTech US will enable BioNTech’s growing presence in the US.
BioNTech US is seeking a passionate, creative, and curious Associate Scientist to join the Biochemistry group. The successful candidate will produce crucial protein reagents to supply many research programs for downstream immunological assay and clinical process development efforts. In addition, the successful candidate will be expected to help develop new biochemical, biophysical, and cell-based technologies and assays in the field of MHC antigen presentation and T cell-mediated immunity.

Here, you’ll achieve greatness.

Primary responsibilities include:
  • Expression of recombinant proteins in E. coli and eukaryotic (predominantly mammalian) systems.
  • Purification of proteins from eukaryotic cultures by affinity, ion exchange, and size exclusion chromatographic techniques.
  • Design and management of DNA constructs for protein expression, purification, and assay development purposes.
  • Develop and optimize cellular expression systems (e.g. E. coli, mammalian cells, insect cells, and yeast).
  • Development of biochemical and cell-based assays to assess TCR binding and functionality.
  • Establishing and cultivating stable mammalian cell lines expressing various biologics.
  • Design, generation, and production of lentiviral constructs and reagents.
  • Labeling (e.g. with biotin or fluorophores) of purified proteins to generate reagents for downstream assays and applications.
  • Maintain cell lines for downstream applications.
  • Improvement of current processes/protocols and development of new technologies.
  • Analysis and presentation of results to larger scientific groups in the company.
  • Productivity both as part of a team and as an independent scientist such that milestones are accomplished against timelines.
  • Keep a very well documented electronic notebook.

What you have to offer.

  • MS in biochemistry, biotechnology, cell biology, or any other molecular biological or relevant biomedical science.
  • At least 3 years of relevant research and/or pharmaceutical/biotechnology industry experience.
  • The successful candidate will need to effectively work both as part of a team and as an independent scientist.
  • Ability to test many different and developing molecular and assay technologies in diverse scientific areas, both at low and high throughputs.
  • Deep hands-on experience with and a solid theoretical understanding of protein expression in bacterial and mammalian cell systems.
  • Proficiency in various protein purification techniques, such as affinity, ion exchange and size exclusion chromatographic approaches.
  • Protein characterization techniques, such as SDS-PAGE, Western blot, ELISA, and analytical HPLC.
  • Experience in subculturing of mammalian cell lines requiring aseptic techniques (such as proper biosafety cabinet operation).
  • Hands-on experience with and a solid theoretical understanding of modern molecular biology and nucleic acid cloning techniques.
  • Familiarity with AKTA purification and HPLC systems is strongly preferred.
  • Excellent organizational and communication skills.
  • Pro-active mentality.
  • Excellent attention to detail.
  • Ability to maintain inventories of equipment, supplies, and related research materials.
Preferred Requirements:
  • Experience with and understanding of biochemical and biophysical binding assays (e.g. TR-FRET, FP, SPR, BLI).
  • Experience implementing automation and liquid handing into protein expression and purification processes.
  • Experience with next generation sequencing technologies and/or data analysis is a plus.
  • Proficiency in flow cytometry analysis of engineered and primary cells (6 colors or more) is a plus.

Benefits for you.

BioNTech US is committed to the well being of our team members and offers a variety of benefits supporting our diverse employee base. Salaried/Position-Targeted Hourly Employees working 30+ hours per week are eligible for our comprehensive benefits package. Benefits include but are not limited to:
  • Medical, Dental and Vision Insurance
  • Life, AD&D, STD and LTD Insurance
  • HSA & FSA Spending Accounts
  • Health & Wellness, including free onsite gym access
  • Adoption & Surrogacy Assistance
  • Vacation and Unlimited Sick Time
  • Holidays and Floating Holidays, including discretionary winter shutdown
  • 401(K) Plan with Significant Company Match
  • Tuition Reimbursement and Professional Development
  • Commuting Assistance and subsidized parking
  • Discounted Home and Auto Insurance
  • Pet Insurance
Plus more benefits that will be shared upon hire!

Have we kindled your pioneering spirit?

Then apply now for our location Cambridge (Boston) and simply send us your application documents using our online form.

For those who require assistance due to disability, or have questions prior to applying, please email

*BioNTech does not differentiate on the basis of gender, race or ethnicity, religion, color, sexual orientation or identity, disability, age and other protected statuses as given by applicable law. We are committed to creating a diverse environment and are proud to be an equal opportunity employer. Most important – it’s a match!