Scientist, Proteomics

  • BioNTech Careers
  • Cambridge, Massachusetts
  • Work experience
  • Research, development, teaching
  • Published: 13.12.2022
  • published till: 17.02.2023
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Scientist, Proteomics

BioNTech is a Germany-based biotechnology company with U.S. offices in Cambridge, MA and Gaithersburg, MD, that focuses on developing cancer therapeutics, including individualized immunotherapy, as well as vaccines for infectious diseases, including COVID-19. The company’s oncology pipeline contains several classes of drugs, including mRNA-based drugs to encode antigens, neoantigens, cytokines, and antibodies; cell therapies; bispecific antibodies; and small-molecule immunomodulators. BioNTech is partnered with several large pharmaceutical companies, including Roche, Eli Lilly, Pfizer, Sanofi, and Genmab. Comirnaty (COVID-19 vaccine) is its first commercialized product.
 
BioNTech is seeking a highly motivated Scientist to join our Proteomics team. The successful candidate will work independently and as part of a team to help develop, implement and apply state of the art proteomics technologies for the detection, identification and quantification of naturally processed and presented peptide antigens across oncology and infectious disease programs.  The candidate will be part of a cross-functional team made up of immunologists, biochemists, clinicians and computational biologists whose goal is to design and contribute to novel immunotherapies.
 

Here, you’ll achieve greatness.

Responsibilities:
  • Sample preparation for nanoLC-MS/MS analysis, including desalting, chemical modification, fractionation, and isobaric tag labeling
  • Optimization and execution of immunopurification and other enrichment strategies
  • Generation of biological samples via mammalian cell culture
  • Method development and implementation of protein purification strategies for isolation of membrane and intracellular proteins from cells and tissues
  • Routine instrument maintenance and benchmarking (Thermo and Bruker instruments)
  • nanoLC-MS/MS analyses of biologically derived protein and peptide mixtures
  • Analysis of nanoLC-MS/MS data and post-processing of data
  • Contributions to nanoLC-MS/MS experimental design
  • Review of scientific literature and collaboration with cross-functional teams
  • Tracking and compiling of results in an electronic notebook
  • Assisting with training of lab personnel
  • Presentation of results to cross-functional teams

What you have to offer.


Requirements:
  • PhD degree or equivalent industry experience (6+ years) in proteomics, mass spectrometry, biochemistry, and/or analytical chemistry
  • Hands on experience with nanoLC-MS/MS workflows for data acquisition including unbiased (e.g. DDA, DIA) and targeted (e.g. PRM, IS-PRM) data collection and MS-based quantitative assays, and ability to modify such methods
  • Background in biochemistry, cell biology, molecular biology, biotechnology and immunology
  • Experience with mammalian cell culture, including transfection and lentiviral transduction
  • Strong familiarity with proteomic wet-lab protocols (immunoprecipitation, enzymatic digestion of proteins, chemical modification of peptides such as iTRAQ/TMT, desalting, fractionation, isobaric tag labeling, etc.) and ability to troubleshoot/modify such protocols.
  • Familiarity with biochemical purification techniques, such as solid phase extraction, ultracentrifugation, and chromatography
  • Experience with data processing workflows, such as database searching programs (e.g. Proteome Discoverer) and quantitative software (e.g. Skyline) or equivalent software
  • Excellent attention to detail, organization, and record-keeping skills
  • Ability to maintain inventories of equipment, supplies, and related research materials
  • Ability to work independently as well as part of a collaborative, matrix environment
  • Independent, flexible, self-motivated, curious, and the ability to work well with others
Optional skills:
  • Exposure to R or other statistical programming tools
  • Exposure to software for DIA analysis (Spectronaut, DIA-NN, EncyclopeDIA, Skyline, or equivalent)

Benefits for you.

BioNTech US is committed to the well being of our team members and offers a variety of benefits supporting our diverse employee base. Salaried/Position-Targeted Hourly Employees working 30+ hours per week are eligible for our comprehensive benefits package. Benefits include but are not limited to:
  • Medical, Dental and Vision Insurance
  • Life, AD&D, STD and LTD Insurance
  • HSA & FSA Spending Accounts
  • Health & Wellness, including free onsite gym access
  • Adoption & Surrogacy Assistance
  • Vacation and Unlimited Sick Time
  • Holidays and Floating Holidays, including discretionary winter shutdown
  • 401(K) Plan with Significant Company Match
  • Tuition Reimbursement and Professional Development
  • Commuting Assistance and free onsite parking
  • Discounted Home and Auto Insurance
  • Pet Insurance
Plus more benefits that will be shared upon hire!

Have we kindled your pioneering spirit?

Then apply now for our location Cambridge (Boston) and simply send us your application documents using our online form.

For those who require assistance due to disability, or have questions prior to applying, please email careers@join-us.biontech.de.

*BioNTech does not differentiate on the basis of gender, race or ethnicity, religion, color, sexual orientation or identity, disability, age and other protected statuses as given by applicable law. We are committed to creating a diverse environment and are proud to be an equal opportunity employer. Most important – it’s a match!


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